Self-isolation? Why not, self-propagation?
Mary W.
4 years ago
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Mary W.
3 years agoRelated Discussions
Selfing?
Comments (17)Calvin This is a funny coincidence. The lab I started in was the main level 3 biocontainment facility at UCLA at that time, and we did the bulk of the HIV work then with a particular focus on IL-6. PCR and confocal microscopy was just getting going and I missed out on helping to set up a PCR facility there because I got distracted by ion channels, and moved over to work with a guy doing patch-clamp EP studies of voltage gated ion channels in rat myoblasts. I ran the tissue culture and did (tried to do) the mRNA separation and characterization hoping to find expression of a particular calcium channel and evaluate its role in differentiation of the myoblasts into myotubules. Canada had looked at NPs quite some time ago, and the impression from all was overall very favorable, but the whole thing got squashed in the end in spite of the benefit and the movement never went anywhere. I remember bit of this because the paper reporting on that was one of the references I used when I sat for my comps. I have a confession I once could not part with a bunch of media for my orchids, but did not want to re-use it without some processing, so I bagged up the lot of it and used my old manual steam autoclave that is still in the basement of my clinic and sterilized the batch in 2 loads. I have a new automatic steam system for instruments so I was not worried about basically trying to sterilize dirt in the autoclave I use for surgical instruments, what I had not counted on was the rather unique aroma produced by autoclaving a bark-CHC mix, and how long it might linger in the building. Happily it is not an offensive aroma, very earthy to be sure and it only was noticeable for a few days. I was able to quash any questions about the aroma without being implicated. Good luck to you in your program. Richard...See MoreComputer-based mostly-self-contained intermittent mist system
Comments (3)I chose Slackware because I've used it for most of about 15 years. Once its set up, I don't usually have to fool with it. (That said, once I plugged in my x10 module and started switching the solenoid on and off, I'd not be able to get into the system. I believe it was still operational, but there is apparently some memory leak in the usb system or the x10 application that makes it so that I can't connect. As a result, I've set the Raspberry Pi to reboot every 3 days-ish. I'll let it run like this until I have time to replace the x10 part of the system with a PiFace module I've bought and not had time to dive into. Also, I've had 900+ days of uptime on (a different box running) Slackware before, so I'm confident the problem is in one of the programs in use.) It turns out that I got started too late in the year to have much success, ...and when I did try, I'd set myself up for failure by going out of town the VERY NEXT morning after I'd set this up. Alas, my neighbor who was checking in on things missed the instruction for this system so... I've iterated away from the spray tank and its need of constant filling and pressurization. I've connected garden hose to the solenoid w/ a connector that can be had @ Lowes (garden hose to pipe thread). Its now truly set and forget. I'm able to access the device remotely via ssh anywhere I have internet. Its also set as a webserver, but I don't have current data being displayed any longer. I was graphing temperature and humidity in my greenhouse as well as the closest weather station. It wouldn't be hard to build some sort of webpage to control it, but that's not exactly my strength (...but might make an interesting project). As far as calibration, I spent about 2 weeks calibrating it. Its "offness" wasn't linear and the output from the tool provided by the author of the software didn't seem to handle it much better. In an effort to get a usable product, I've scrapped the temperature/humidity sensor controlled parts until such time as I can get better data from them. (This might mean new sensors, or more refinement in calibration.) The system is now only using time as input into when it should mist. I think its set for 10 seconds every 2 hours at present. I managed to get a few Japanese Maple, Juniper, and Crabapple cuttings to root, and I've got a flat of ~90-ish gardenias under mist right now. Hopefully next year I can get started early and in earnest ...and really put this system through its paces. My primary concern is in the finding of interesting stock to root. Also, if a copy of any of Dirr's propagation manuals comes around I might see about acquiring it. I still need to contain the guts of this system in a project box of some sort for a few different reasons. If I can find the time, I may build out benches for my greenhouse ...and see about installing a heating cable....See Moreminimum population for eggplant/selfing species?
Comments (3)I'm going to say that you will be fine. Inbreeding depression is a generational thing. Think of it like rolling dice, but each time, a number is removed. Your first roll, chances are you'll be okay, you might end up with a 5 and 6 or double sixes even. But, the more times you roll, the higher your chances are of getting snake eyes. that a *little bit* like inbreeding. The first time someone in the family kisses their cousin, there's a good chance their kids will turn out perfectly normal. If they make a habit of it though, and each generation continues to kiss cousins, the chances of potentially dangerous (or maybe just odd) recessive genes increases. With plants that are self fertile and self pollinating, there are biological mechanisms in place to help stave off this dilemma. Otherwise, there would be no self fertile plants (adaptation/evolution would have weeded them out eons ago). So, they can happily breed with themselves time after time and the ill effects will be much longer in the making. That doesn't mean they don't benefit from having a healthy population to cross with, but, if they don't, chances are high you wont find them much worse for the wear. While it may be minimal, there is still going to be *some* gene recombination and the offspring are not clones of the parent. Although, at some point, I would order more seeds to introduce some variation and maintain long term vigor, it's not, in my mind, a pressing issue that must be done this instant, just at the earliest convenience....See MoreSay a prayer--I'm about to use some Ardex-self leveler!!
Comments (25)Glenn and Nick--you guys have been so nice and encouraging and helpful! I wish you lived nearby--I'd bake you a cake or make you a great steak dinner or somethin'!! :o) Yep, I will not be happy with this floor till I do some of that Henry's fill! We have one more bag of the Ardex left, and I've toyed with putting it down and hoping it will fix the slight inequities but---the stuff that has been down since yesterday around 1:30 p.m. still 'looks' uncured, enough, to me (the novice) to feel safe putting a moisture barrier on and start laying the planking down. If it's taking so long to cure then we'd be out another couple of days and we will have lost our window of opportunity to have our one SIL helps us lay the floor (he's working off a loan to do it) even though I feel very confident I could do that part on my own with hubby making all the cuts! We just need to let him do this! :o) So, I'm glad to hear I can just put 'Henry' over the Ardex without priming--I had guessed that might be the case but I also thought I might have to prime the Ardex that's down, in order to put more Ardex on top, so that would add to the delay considerably, as well, and then if the floor indeed is not completely cured, I might not should put the primer on it, just yet--which again....etc.!! So, trying to use some logic--I guess it's good ole' Henry! :o) I so appreciate the support you guys have given me, again, and I heartily thank you, again!!!! :o) We need to save as much money as we can on this project, given the 60%+ loss everyone invested have lost, and had counted on to make retirement more 'doable' and without your help we might have had to forget the flooring we have, sell it to our daughter and SIL and gotten carpet, which would still have been more expensive than the deal we got on this stuff!!! Sigh--if we were just starting out with this project, and didn't have the flooring from last summer, we would not have undertaken doing it at all, at this time!!!...See Moresmb1_sunshinegg
3 years agoMary W.
3 years agoDave in NoVA • N. Virginia • zone 7A
3 years ago
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