From that site: Due to the destructive effects Cuban treefrogs have on native species of amphibians and reptiles, many biologists recommend that Cuban treefrogs be euthanized/killed. That's harsh, but true. We recommend a humane method of euthanasia for amphibians. Just make SURE the frog you have is a Cuban treefrog before euthanizing it (see the test suggested below). A humane method for euthanizing amphibians: To euthanize a Cuban Treefrog, hold the frog firmly in your hand and apply a 1 inch bead of benzocaine ointment along the back of the frog. Benzocaine ointment is a topical anesthetic (a numbing agent) used to treat skin pain (e.g., from sunburn) and itching as well as toothaches and sore throats. There are a variety of name brand and generic versions that are available over-the-counter in a tube or spray. If you are able to, using a gloved finger spread the ointment out on the frog's back. Alternatively, you could use a benzocaine spray. Once the ointment or spray is applied, place the frog in a plastic grocery bag or a sealable sandwich bag for 15-20 minutes so that the benzocaine has a chance to render the frog unconscious (be sure to seal the bag or tie it closed). After the bezocaine has anesthetized the frog, place the bag in a freezer overnight to ensure that the frog is dead and then throw it out in your trash. If you are unable to apply benzocaine to the frog, you can simply put it in a plastic bag, seal or tie the bag shut, then place it in the freezer overnight--dispose of the bag and the frog in the trash the next day. Do not throw a bagged frog into the trash without euthanizing it first. Remember, Cuban Treefrogs have a noxious skin secretion so be sure to wash your hands thoroughly after you handle the frog, even if you wear gloves or use a plastic bag. Freezing is a humane way to kill amphibians because their bodies go into a state of torpor (metabolism slows way down) -- just as they do in cold weather outside. If the cold weather is short in duration, the frogs will come out of their torpor state. However, after an extended time in freezing temperatures, the frogs die. A good test to determine if a frog is a Cuban treefrog is to grasp the frog firmly, but gently, and try to move the skin around on the top of the frog's head with your fingertip. The skin on the head of a Cuban treefrog is fused to the top of the skull and won't move. Be sure to wash your hands after handling any frog or toad. They all secrete a slimy film to protect their skin, but the secretions of some species, like the Cuban treefrog, can irritate the skin and eyes of some people.

We do a weekly inspection of all the places they like to hide, and nothing yet. We are HOPING the cold did kill them all. This weekends inspection for the cubans was NONE. We have noticed more tiny green frogs, which is good. PLEASE REPORT any sightings (number and location) to the Professor who does research on frogs and invasive frogs such as the Cubans to: Steven Albert Johnson at tadpole@ufl.edu http://ufwildlife.ifas.ufl.edu/ This site gives you instructions on how to humanely dispose of the cubans. Here is a link that might be useful: University of Florida Wildlife Research

From the UF website : You can reduce the negative impacts of Cuban Treefrogs by eliminating eggs and potential breeding sites. Monitor ornamental ponds or neglected pools for Cuban Treefrog egg masses during the spring and summer, especially after heavy rains. Be sure to check for eggs the morning following rain so the eggs do not have time to hatch. Use a small-mesh aquarium net to scoop out masses of Cuban Treefrog eggs floating on the surface of the pond or pool and simply discard them on the ground to dry out.

We have a veritable orchestra of Cuban Tree Frogs frolicking under our bedroom window. The vocal evidence of amphibious love is deafening at 2am!! While I do NOT want to obliterate them, I would LOVE to discourage them from using this garden space as their little private Club Med. Help, anyone??????