Colchicine use in tissue culture.
Proteus
12 years ago
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cody_mi
12 years agokeking
11 years agoRelated Discussions
Tissue Cultured Plants
Comments (16)As regards to 'mutants', many of the newer hosta varieties are in fact mutants created in TC vats which are multiplied or sale by further TC. This is not to say that there is anything bad about them, as most of the old varieties were mutants created in nature. Mutants occur in TC fairly commonly and are generally rogued out as the plants are raised to production size. A fair number of these are generally kept and evaluated as for improvements over the base stock but most are destroyed in the long run. The reason they occur so commonly is that TC uses such small explants to form each plant that only a very small number of cells must mutate to give a totally mutant plant. If the Big Sky series has a fairly high number of non conforming plants entering the marketplace, then it is a problem of poor control in the growing out step that is at fault, not the TC itself, these should be caught as the plants are being up-sized. George...See MoreTissue Culture and the resulting Own Root Roses
Comments (9)Tissue culturing virused stock will produce virused plants. But there are special techniques used to produce virus-free plants that involve tissue culturing the apical meristem while growing the plant under high heat. That's where nurseries get their guaranteed virus-free roses, and they're more expensive and there's less available because of the extra work involved. Lots of your nursery plants are propagated by tissue culture, including roses. Kalmias in particular are almost impossible to propagate commercially any other way. I used to work at the very very large rhododendron production nursery here, and all their plants are produced by tissue culture - rhodies, azaleas, viburnums, hydrangeas, kalmias, weigelia, clematis, heathers, roses ... the plants are tiny when they come out, they're potted 16 to a 4" pot, but they're grown on to a 2" pot size and then sold to regrowers, nurseries that take the 2" pot plants and grow them on to retail size and then sell them to retailers. A few bargain mail order nurseries buy the 2" pot plants and sell them directly. I do not recommend 2" pot plants for the average home gardener! they require a lot of TLC to keep them growing, just like any other baby....See Morefern tissue culture
Comments (6)Allow me to throw the first snowball. Are you using Gamborg's medium without MS salts and sucrose? I have a recipe for Nephrolepsis media that includes 1/2 MS salts, 20g/L sucrose, 0.1 mg/L of NAA and 1.0 mg/L of Kinetin. If you are using all of these you might try different concentrations of hormones to find one that works best for the species you are trying to grow....See MoreWANTED: For Canadians: another hosta tissue culture group ?
Comments (3)Hi Digs, Well, I am surprised to see you post about placing an order after I wrote to ask you for the information in order to place an order myself . Is that what spurred you to do it ? LOL !! I might be interested but most of the hostas you have listed are small or minis with only one or two mediums and I am interested more in large or giant hostas .....but .....I will say yes tentatively and wait and see what happens. Signet...See Morechara2
11 years agokeking
11 years agochara2
11 years agokeking
11 years agochara2
11 years agodavidrt28 (zone 7)
11 years ago
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