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henry_kuska

High throughput sequencing (HTS) as rose virus detection technique

henry_kuska
3 years ago

At the July 22, 2020 meeting of the National Clean Plant Network (NCPN) - roses a paper was presented that compared a new technique High Throughput Sequencing (HTS) with three of the earlier techniques used to detect viruses in roses at the University of California at Davis.


https://ucanr.edu/sites/ncpnrose/files/330324.pdf


One of the earlier test is called a Multiflora test. On slide 12 it was reported to be correct 77% of the time (17 plants), but gave a false negative 23% of the time (5 plants).


Another early test is called a Shirofugen test. On slide 15 it was reported to be correct 23% of the time (5 plants), but gave a false negative 77% of the time (17 plants).


See also slide 17 and slide 19. Slide 19 is of particular interest as it indicates that even the relatively modern PCR test can give false negative tests.

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