and here is a quite interesting and complementary piece: [Date Prev][Date Next][Thread Prev][Thread Next][Date Index][Thread Index] Cultivating diversity underground for better yields above - http://www.newfarm.org/depts/NFfield_trials/0903/daviddouds.shtml * To: lfl@intrex.net * Subject: Cultivating diversity underground for better yields above - http://www.newfarm.org/depts/NFfield_trials/0903/daviddouds.shtml * From: "Lawrence F. London, Jr." * Date: Mon, 12 Apr 2004 23:24:01 -0700 * Delivered-to: lfl@intrex.net * Organization: http://market-farming.com -- Venaura Farm * User-agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.5) Gecko/20031007 http://www.newfarm.org/depts/NFfield_trials/0903/daviddouds.shtml Cultivating diversity underground for better yields above Research at The Rodale Institute® demonstrates how sustainable farming practices can boost yields by nurturing beneficial soil fungi. By Laura Sayre About this series: As some of you may know, The Rodale Institute®, which publishes The New Farm®, is home to the longest running field trials in the country comparing organic and conventional systems of farming called The Rodale Institute Farming Systems Trial® (FST). The data from that 23 years of research is a real treasure trove of insight into the economic, ecological and agronomic benefits of organic farming. In addition to this long-running Farming Systems Trial, we have a variety of other research in progress at The Institute. David Douds, as youll read in this story, has been studying soil fungi here at The Institutes research farm for 15 years. Were engaged in no-till research, weed research, compost tea research, composting research, water quality research, and much more. Until now, much of the light were generating here on our research farm has been hidden under the proverbial barrel, but were taking off the barrel and busting it up for firewood. Were going let the light of the amazing research being done here shine on farmers, consumers and environmental activities. Over the next year well be running a series of stories, about one a month, on the significance of our research ... and its practical applications. That includes a few stories on equipment constructiona front-mounted roller for no-till, and a compost turner converted from a junked 18-wheeler. So sit tight, and be prepared to be amazed, starting with David Douds discoveries about how you can increase vegetable yields by 50 percent using homemade fungal inoculants. Enjoy, Chris Hill Executive Editor p.s. Interested in hearing more about how you can take part in the mycorrhizae revolution? Click here and let us know. Send your name, phone number and e-mail address with your note so we can follow-up with you. "Overall, Douds work suggests that a small amount of mixed MF inoculant can be substituted for a large amount of fertilizer--with no loss of yield, greatly reduced environmental impact, and lower production costs." Home-grown mycorrhizal inoculum can be produced at a fraction of the cost of purchasing commercial mixes. "I've done some preliminary calculations," says Douds. "The on-farm system produces 100 million propagules for approximately $50, not counting the cost of the farmer's labor, which is fairly minimal. To purchase 100 million propagules as listed on the bag of some commercial mixes would cost anywhere from $8,000 to $40,000." FACT SHEET: Mycorrhizae Above: USDA soil microbiologist David Douds with a carrot root-mycorrhizal fungi culture. Under the leadership of Dr. Douds, field trials have shown yield gains of as much as 50% in the presence of healthy mycorrhizae populations. Now Douds is developing a practical, low-cost method for on-farm production of mycorrhizal soil innoculant, promising higher yields with lower nutrient inputs. (Photo by Peggy Greb, courtesy of the Agricultural Research Service Photo Unit.) September 29, 2003: You've read the amazing facts and figures: one teaspoon of healthy topsoil can contain millions of individual microorganisms, all playing a part in the functioning of the soil ecosystem. But how much do you really know about the action of those diverse species and how to maximize their presence in your own fields? Ongoing research at The Rodale Institute® sheds light on one important component of the soil community--mycorrhizal fungi--and its impact on agricultural production. Under the leadership of Dr. David Douds, a soil microbiologist with the USDA's Agricultural Research Service, field trials have shown yield gains of as much as 50% in the presence of healthy mycorrhizae populations. Now Douds is developing a practical, low-cost method for on-farm production of mycorrhizal soil inoculant, promising higher yields with lower nutrient inputs. A mycorrhizal primer Mycorrhizae are soil-dwelling fungi that live in and around the roots of plants ('myco-rrhizae' means 'fungus-root'). The fungi and the plants form mutually beneficial associations in which the fungi receive carbohydrates from the plants and the plants receive nutrients and other benefits from the fungi. Since the first mycorrhizae species were described by a German botanist in the 1880s, researchers have discovered that approximately 80% of all land plants form mycorrhizal associations. The relationship is so widespread, in fact, that it is sometimes referred to as 'the Universal Symbiosis,' and is believed to have played a key role in the evolutionary transition from aquatic to terrestrial plant forms. Today, scientists divide mycorrhizae into two major types: endomycorrhizae, which penetrate and colonize plant roots, and ectomycorrhizae, which form sheaths around plant roots. Whereas ectomycorrhizal relationships tend to be highly specialized--with some 6000 fungal species worldwide associated with tree species of the oak, beech, and pine families, among others--endomycorrhizal associations are more generalized as well as more widespread, with fewer than 150 fungal species opportunistically colonizing the roots of the vast majority of terrestrial plant families. Ectomycorrhizal inoculants are already widely used in commercial forestry, but the possibility of developing endomycorrhizal inoculants for production agriculture is a more recent idea. "These are beneficial soil fungi that colonize the roots of plants and help them take up phosphorus" and other immobile soil nutrients, such as zinc and copper, Douds explains. "The fungus colonizes the root and it also grows out into the soil; the part of the fungus that's in the soil acts as an extension of the root system, to explore a greater volume of soil and take up nutrients and bring them back into the root." In addition to facilitating nutrient uptake, some mycorrhizae secrete a gluey substance, called glomalin, which helps develop soil structure and soil aggregation; others may help plants fight disease. Yet because endomycorrhizae are 'obligate symbionts'--they must have living plant roots to colonize in order to complete their life cycle--their numbers will decline under conventional agricultural monocultures, which have living crop covers fewer months of the year than organic rotations. The drop in yields typically seen after the first year of cultivation on virgin prairie or forest soils is probably attributable in part to the loss of native mycorrhizae, Douds says. Fifteen years of research prove benefits of fungi Douds has been conducting research in collaboration with The Rodale Institute since 1989, his first year at the Agricultural Research Service's Eastern Regional Research Center in Wyndmoor, on the outskirts of Philadelphia. "Some employees of The Institute farm came down to our research center as part of a kind of an interagency show-and-tell about research programs and facilities and what all we could do to help each other," Douds recalls. "Rhonda Janke"--The Institute's research agronomist at the time--"gave a presentation about The Rodale Institute Farming Systems Trial," a side-by-side comparison of organic and conventional production systems. Douds recognized it as a great opportunity to study endomycorrhizal associations. "Later that year I started sampling, and right off learned that [the soils under] the conventional farming systems had fewer mycorrhizal fungi than the soils under the low-input farming systems. So right away we all got excited and we branched out from there." Since that first season, Douds' work at The Rodale Institutes 333-acre experimental farm has progressed in three overlapping phases: In the first phase, from 1989 to 1995, Douds and his team surveyed native mycorrhizal fungi (MF) populations at Rodale and examined the impact of different agricultural practices--including tillage regimes, crop rotations, and soil amendments--on those populations. The second phase, which is still ongoing, looks at the utilization of MF by crop plants, comparing yields in the presence and absence of different MF species. The third phase seeks to apply those findings by devising a simple, on-farm MF inoculum production system, so that farmers can harness the benefits of endomycorrhizae without spending lots of money on commercial mixes. (Commercial products already on the market include Bio/Organics Endomycorrhizal Inoculant [$79.95 for 3 lbs, labeled to treat 500 plants], Plant Success Mycorrhizae Tablets [$19.95 for 100 tablets, labeled to treat 50 plants up to 1 ft tall], and Earthroots VAM Fungi by First Fruits LLC [$15 for 3 lbs, labeled to treat 200 seedlings].) On-farm production of mycorrhizal inoculant in test enclosures at The Rodale Institutes farm. Douds chose bahiagrass as a host plant "because it's a tropical grass and the first frost will kill the shoot growth"--so it won't escape to become a new local weed and won't harbor any pests or pathogens that might affect either resident crops or northern native grasses. Agronomic practices that boostor depress mycorrhizal levels Although the first phase of Douds's research found larger and more diverse MF populations in organically-managed soils than in conventionally-managed ones, it also revealed how specific agronomic practices can boost or depress MF levels. "Over-wintering cover crops. . . are very beneficial to mycorrhizal fungi," Douds notes, whereas "tillage disrupts the mycorrhizal fungi in the soil and serves to decrease the initial colonization of the plants." Based on these findings, Douds emphasizes that all farmers, organic or conventional, can take steps to nurture the MF already present in their fields: reduce tillage, he says, use fungicides sparingly, and--most important--maximize cover cropping. "Over-wintering cover crops give the MF a host plant to colonize when there's no cash crop growing on the soil," Douds explains. In the coldest part of the year the MF go dormant, but during warm spells in early spring and late fall, the MF will try to grow, and can exhaust their reserves if they find no plant hosts. "During these periods. . . the fungus is still respiring, it's still burning up its carbohydrate storage in the spores, it's burning up the lipids that were stored," leaving it "less viable when the time comes finally for the crop plant to be present." A cover crop or even just a weedy fallow will maintain healthy MF populations, which can then benefit the cash crop coming on to the field. Crop rotations are another factor to consider, since a handful of crop species belong to plant families that do not form mycorrhizal associations (said to be 'non-mycotrophic'), including the Brassicaceae (rape, broccoli, cabbage, turnips, etc), the Chenopodiaceae (beets, spinach), and the Polygonaceae (buckwheat). Not only will these crops not benefit from the presence of MF, but MF levels in the soil will be depressed after these crops are grown, potentially showing an effect on any mycotrophic crops which follow. Potatoes and peppers inoculated with mycorrhizae get yield boosts of up to 50 percent! In the second phase of his research, looking at the impact of MF on crop yields, Douds began inoculating plants in the greenhouse and then tracking their performance in the field. "We had some plants that were inoculated with a control mix with no inoculum, another one inoculated with a mix of mycorrhizal fungi, and another inoculated with just one species commonly present in commercial inoculum," Douds explains. "We transplanted them into the Compost Utilization Trial,"--another ongoing experiment at The Rodale Institute--"and we found over the course of the 3-year experiment that the mixture of mycorrhizal fungi increased the yield of marketable-sized peppers up to a maximum of 34% over the control. Last year we tried inoculating potatoes, and we got up to a 50% increase over the controls." David Douds and a research intern dig potatoes in this season's mycorrhizal test plot at the Rodale Experimental Farm. In last year's trials, potatoes grown with mycorrhizal fungi showed yield increases of as much as 50%. Other crops known to respond dramatically to mycorrhizal colonization include citrus, onion, and strawberries. This year they are repeating the potato trial, measuring yields under four different treatments: one with no added MF; one with a commercially available MF; one with a mixed MF inoculant grown in a leaf compost and vermiculite medium; and one with a mixed MF inoculant grown in a dairy manure compost and vermiculite medium. Overall, this work suggests that a small amount of mixed MF inoculant can be substituted for a large amount of fertilizer--with no loss of yield, greatly reduced environmental impact, and lower production costs. One unexpected finding of Douds' work at Rodale "is that mycorrhizae can be used to increase the yield of crops even in soils that are very high in phosphorous." Some of the soils at the Rodale Farm which have been heavily composted, Douds notes, "have available P in excess of 300 parts/million"--well above the level at which mycorrhizal responses are typically seen, around 20-50 ppm available P. "The generalization would be that P as high as 300 would be a situation in which the plant can take up all the P that it needs by itself without relying on the mycorrhizal fungi." Douds believes that at high nutrient levels, some of the other benefits of MF--enhanced disease resistance, improved soil aggregation and better water relations--could be showing an effect. Build your own on-farm inoculum production system The third phase of Douds' research at Rodale Farm focuses on developing an inexpensive, practicable system for on-farm production of mycorrhizae inoculant. As obligate symbionts, endomycorrhizae have so far resisted attempts to create what scientists call axenic (or isolated, single-species) cultures--they can only be grown in the presence of a host plant. Douds' system works within this constraint, using bahiagrass (Paspalum notatum), a tropical grass native to the southeastern US, as a host. A myccorhizae factory: The basic procedure is for the farmer to construct a simple enclosure out of landscape fabric, fill it with a mixture of compost and vermiculite, and then transplant pre-colonized bahiagrass seedlings into the mixture. Over the course of the growing season the bahiagrass spreads within the enclosure and the mycorrhizal fungi spread and reproduce along with it. When the grass dies back in the winter, the farmer is left with a concentrated mycorrhizal inoculant that can be incorporated into his or her potting mix when starting seedlings in the greenhouse the following spring. The basic procedure is for the farmer to construct a simple enclosure out of landscape fabric (75 cm square and 20 cm high), fill it with a mixture of compost and vermiculite, and then transplant pre-colonized bahiagrass seedlings into the mixture. Over the course of the growing season the bahiagrass spreads within the enclosure and the mycorrhizal fungi spread and reproduce along with it. When the grass dies back in the winter, the farmer is left with a concentrated mycorrhizal inoculant that can be incorporated into his or her potting mix when starting seedlings in the greenhouse the following spring. This year, Douds gave inoculated bahiagrass seedlings and other materials to a few Pennsylvania farmers to see how the method fares in the real-life conditions of farming. Meanwhile, Douds has 12 soil enclosures growing at the Rodale Farm in an experimental grid designed to identify optimum growth media. Douds chose three different kinds of compost--yard-clippings compost, controlled microbial compost, and dairy manure-leaf compost--and then diluted each kind with vermiculite at four different ratios, ranging from 1 part compost:2 parts vermiculite, down to 1 part compost:49 parts vermiculite. Each soil enclosure, finally, has nine separate sections, three with no inoculant and three each with two different mixtures of MF. At the end of the season, says Douds, "we'll sample the mixtures from within each enclosure, quantify the inoculum production, and then hopefully develop a prediction formula, where the optimum ratio [of compost to vermiculite] is a function" of the nutrient analysis and other properties of the compost. All the farmer will need to do, then, is get the nutrient analysis of his or her compost, plug it in to the formula, and find the optimal ratio of compost to vermiculite to use for his or her farm. "On-farm methods have several advantages over commercial inoculants," Douds explains. In the first place, whereas commercial formulae typically only contain a single MF species (frequently Glomus intraradices), Douds' method yields a diverse inoculum containing many MF species. This is crucial because MF show significant 'functional diversity'--"some are good at holding the soil together, some are good at gathering nutrients," others help fight disease. A second, related advantage is that by mixing in some soil from a nearby woodland, prairie, or hedgerow, the farmer can use Douds's system "to produce the native or indigenous strains of mycorrhizal fungi. . . the ones that are already adapted to his [or her] particular soil conditions." This could be especially important on problem soils, such as those with high aluminum, say, or high or low pH, where commercially-produced fungi may not survive. Growing fungi in real life: David Douds with one of his on-farm mycorrhizal fungi production systems at Shenk's Berry Farm in Lititz, PA. John Shenk, the cooperating farmer, grows 5 acres of strawberries, 3 acres of raspberries, and 10 acres of mixed vegetables in a low-input system, selling on-farm and at the Clark Park Farmers Market in Philadelphia. "We try to farm thoughtfully," says Shenk. "And do lots of reading and research to keep improving our farming methods." Next season, Shenk will incorporate the soil from the enclosure into his greenhouse potting mix. Last but not least, home-grown mycorrhizal inoculum can be produced at a fraction of the cost of purchasing commercial mixes. "I've done some preliminary calculations," says Douds. "The on-farm system produces 100 million propagules [in a single enclosure] for approximately $50, not counting the cost of the farmer's labor, which is fairly minimal. To purchase 100 million propagules as listed on the bag of some commercial mixes would cost anywhere from $8,000 to $40,000." Commercial inoculants are sold in a peat- or vermiculite-based medium, so purchasers have to buy (and pay to have shipped) a large volume of material to get a small number of viable MF propagules--another reason it makes more sense to grow your own. At the moment, Douds' system (like commercial MF inoculant) is suitable for two types of farms: vegetable growers on any scale who produce their own seedlings and can mix the inoculum into their potting mix; and smaller, labor-intensive farms or urban gardens where "the inoculum can be incorporated by hand, directly into the planting furrow or planting hole." Farmers growing field crops on a large scale can only take advantage of MF inoculants if they want to try them out in a relatively small area. "Delivery of MF inoculum to the field is a problem," acknowledges Douds. "Commercial companies are working on this for their particular inocula." He smiles. We can only hope that he will be too. OPX e-letters to the e-editors news & research columnists gleanings 1000 stories talking shop certification the pig page this farm life newsletters mid-atlantic marketing new farm japan FARM LOCATOR PRESS ROOM Farmers: Is organic certification worth it? 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"biocontrol" is one method that one can try for blackspot. The following may be useful in putting the concept of biocontrol into layman terms: "Disease-suppressive soil microorganisms have been found in many places. In monoculture wheat the severity of "take all" disease often decreases within three to five years. This phenomenon is known as "take all decline," and is considered an effective natural control. Although the mechanisms are not completely understood, the decline is associated with changes in soil microorganisms that compete with and prey on the fungus. Melon plants grown in the Chateaurenard region of France do not show Fusarium wilt symptoms even though the fungus is present in the soil. Soils with suppressive characteristics tend to develop slowly and are usually found in fields where perennial crops or monocultures have been grown for many years. Suppressiveness may be lost if the monoculture is interrupted even for one year, or if pesticides are applied. For example, researchers first recognized soils suppressive to cereal-cyst nematode when nematode numbers increased after application of a broad-spectrum biocide. Many species of fungi and bacteria in the genera Trichoderma, Streptomyces, Bacillus and Pseudomonas suppress diseases, but at this time only a few strains are commercially available. Additional commercial products may be available soon, however, as this is currently an active research area." The quote was taken from: Http://www.cals.ncsu.edu/sustainable/peet/IPM/diseases/org_cert.html ---------------------------------------------- The following article from the Maine Rose Society web page ( http://www.mainerosesociety.com/articles/control.html ) was written by by DR. LAKSHMI SRIDHARAN, who should be familar with those of you that are members of the American Rose Society. ----------------------------------- Examples that friendly fungi do exist: Http://taylorandfrancis.metapress.com/app/home/contribution.asp?wasp=59ghxnlylp03feg2dr2p&referrer=parent&backto=issue,1,10;journal,2,54;linkingpublicationresults,1:100635,1 Http://taylorandfrancis.metapress.com/app/home/contribution.asp?wasp=4g1ltlxhtr3ya223recn&referrer=parent&backto=issue,8,10;journal,4,54;linkingpublicationresults,1:100635,1; Http://taylorandfrancis.metapress.com/app/home/contribution.asp?wasp=8000375kwm5qnk5e7y47&referrer=parent&backto=issue,1,10;journal,6,54;linkingpublicationresults,1:100635,1; Http://taylorandfrancis.metapress.com/app/home/contribution.asp?wasp=07wckmqrxr3jqg6kducy&referrer=parent&backto=issue,9,10;journal,7,54;linkingpublicationresults,1:100635,1; ------------------------------------------------ Blackspot on roses is scientifically called "diplocarpon rosae". The link below reports that trichoderma harzianum is a promising control - see their exact wording (the document is a PDF document. The applicable section is easily found by typing diplocarpon into the PDF find command - the binoculars). Http://www.gtz.de/ecosan/download/Bangalore03-Ramanujam.PDF ------------------------------------------------------ Title: The Abundance and Structure of the Root-Associated Microbial Complexes of Two Greenhouse Rose Cultivars. Authors: Polyanskaya, L. M.; Ozerskaya, S. M.; Kochkina, G. A.; Ivanushkina, N. E.; Golovchenko, A. V.; Zvyagintsev, D. G. Authors affiliation: Faculty of Soil Science, Moscow State University, Vorob'evy gory, Moscow, Russia. Published in: Microbiology (Moscow, Russian Federation)(Translation of Mikrobiologiya), volumn 72, pages 496-502, (2003). Abstract: "The study of the root-assocd. microbial complexes of affected and healthy rose plants of two cultivars (Grand gala and Royal velvet) grown in a greenhouse showed that the biomass of eukaryotic microorganisms in the rhizoplane and rhizosphere of healthy rose plants and in the surrounding soil was considerably lower than in the same loci of affected plants. In contrast, the biomass of root-assocd. prokaryotic microorganisms was higher in the case of healthy than in the case of affected rose plants. The root-assocd. bacterial complexes of both affected and healthy rose plants were dominated by the genera Arthrobacter, Rhodococcus, and Myxobacterium and did not contain phytopathogenic bacteria. The root-assocd. fungal complex of healthy roses was dominated by fungi of the genus Trichoderma, whereas that of the affected rose plants was dominated by the species Aureobasidium microstictum. The affected cane cuttings and cankers occurring on affected canes were found to contain Coniothyrium fu-ckelii (the causal fungus of rose stem canker) and sclerotia of Botrytis cinerea (the causal fungus of gray rot). The micromycete complex of healthy rose plants was not so diverse as was the micromycete complex of affected rose plants." ---------------------------------------------- Title: Biological control of black spot of rose caused by Dipocarpon rosae . Authors: Prasad, R. D.; Rangeshwaran, R.; Sunanda, C. R.; Vinita, J. Authors affiliation: Project Directorate of Biological Control, Post Bag No. 2491, H.A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, India. Published in: Annals of Plant Protection Sciences,volumn 10, pages 256-259, (2002). Abstract: "Fungal biological control agents (Trichoderma harzianum , T. viride and Chaetomium globosum ) were used either alone or in combination with fungicides (chlorothalonil and mancozeb) to manage black spot of rose caused by D. rosae under greenhouse conditions. Black spot incidence in biological control agent and/or fungicide treatments was significantly low (disease ratings from 0.33 to 3.33) compared to the control at all observation dates. After 100 days of spraying, defoliation was lowest with chiorothalonil, Trichoderma harzianum +chlorothalonil, C. globosum +chlorothalonil and T. harzianum +mancozeb treatments. The highest mean vigour index was recorded in T. harzianum treatment. The highest flower production was recorded in C. globosum +chlorothalonil treatment (4.33) followed by T. harzianum alone and T. harzianum +chlorothalonil treatment (4.00)." -------------------------------------------------- Cornmeal is considered to be a good food for the friendly fungus "trichoderma harzianum". The fungus is available commercially, see: http://www.ipmofalaska.com/files/trichoderma.html There may be another benefit to this fungus as there is a report that it helps root growth: Title: Effect of Trichoderma Colonization on Auxin-Mediated Regulation of Root Elongation. Author: Bjoerkman, Thomas. Department of Horticultural Sciences, Cornell University, Geneva, NY, USA. Published in: Plant Growth Regulation, volumn 43, pages 89-92, (2004). Abstract: "The biocontrol fungus Trichoderma harzianum 1295-22 increases root growth in addn. to roles in suppressing disease. Its agricultural use could be expanded if the mechanism of growth enhancement were known. Among the proposed mechanisms of growth enhancement is that the fungus counteracts auxin inhibition of root-cell elongation. We tested whether there was evidence for a secreted auxin inhibitor, for enhanced auxin degrdn., or for altered auxin sensitivity. Our results provide no support for any of these mechanisms. Trichoderma secretions inhibited growth, whereas an auxin inhibitor would increase growth. Auxin inhibited growth to the same extent in colonized and uncolonized roots, indicating no change in auxin sensitivity. Endogenous auxin levels maintained growth closer to the max. in uncolonized roots, indicating stronger auxin limitation of growth in colonized roots. These tests indicated that Trichoderma-colonized roots had a faster max. growth rate, but an unchanged response to auxin." -------------------------------------------- Even if naturally occurring trichoderma harzianum only reduces the amount of blackspot fungus by 10 to 20 % (the numbers are just a guess), there are probably going to be a number of different "friendly" fungi in an organic garden. For example, the following paper reports that 2 fungi that are now recognized as biocontrol potential have increased levels on blackspot infected leaves: ------------------------------------ Title: PHYLLOPLANE MICROORGANISMS OF ROSA CULTIVAR PICADILLY FOLLOWING INFECTION BY DIPLOCARPON-ROSAE Author: HAYES A J Author Affiliation: DEP OF FORESTRY AND NATURAL RESOURCES, UNIV OF EDINBURGH, UK. Published in: Transactions of the British Mycological Society, volumn 79, pages 311-320, (1982). Abstract: "The phylloplane microflora of the hybrid tea rose cultivar Picadilly was studied for 2 growing seasons on healthy leaves and on leaves infected by D. rosae. Large increases in numbers of yeasts and bacteria on healthy leaves were noted from the end of July until the beginning of Oct. Late in the growing season numbers of all types of microbes increased dramatically. Following infection by D. rosae numbers of microbes generally showed a marked increase, but this was not always sustained. Cryptococcus laurentii and Micrococcus sp. populations on infected leaves were 3-4 times those on correspondingly healthy leaves. The species composition and changes in numbers of phylloplane microbes are compared with descriptions of microflora isolated from leaves of other plants and possible reasons for the observed differences are discussed." -------------------------------------- The following two links are Google searches for the two fungi listed and the word biocontrol. --------------------------------------- Http://www.google.com/search?hl=en&lr=&q=%22cryptococcus+laurentii%22+biocontrol&btnG=Search ----------------------------------------------- Http://www.google.com/search?hl=en&lr=&q=micrococcus+biocontrol&btnG=Search ------------------------------------------- The science of biocontrol is still in its infancy. The use of Chitosan is one of the early results of this area of research. The following is the EPA summary: ( Http://www.epa.gov/pesticides/biopesticides/ingredients/factsheets/factsheet_128930.htm ) "SUMMARY Chitosan is used primarily as a plant growth enhancer, and as a substance that boosts the ability of plants to defend against fungal infections. It is approved for use outdoors and indoors on many plants grown commercially and by consumers. The active ingredient is found in the shells of crustaceans, such as lobsters, crabs, and shrimp, and in certain other organisms. Given its low potential for toxicity and its abundance in the natural environment, chitosan is not expected to harm people, pets, wildlife, or the environment when used according to label directions." -------------------------------------------- There is a scientific literature report of 10 - 20 % effectiveness against blackspot. Title: Chitosan as the biocontrol agent of fungal pathogens; activity and mode of action. Authors: Wojdyla, Adam T. Authors affiliation: Research Institute of Pomology and Floriculture, Skierniewice, Pol. Published in: Bulletin of the Polish Academy of Sciences: Biological Sciences, volumn 51, pages 159-165, (2003). Abstract: "In in vivo expts. showed that chitosan added to potato dextrose agar at 3 mg/cm3 in 50% suppressed radial growth of Colletotrichum gloeosporioides and Phytophthora cryptogea. Another tested fungi were less sensitive to chitosan. ED50 for Cylindrocladium scoparium and Myrothecium roridum was more than 5 mg/cm3. In vivo expts. showed that only 10-20% effectiveness of the compds. against Diplocarpon rosae was found, when chitosan at 0.2-0.4 mg/cm3 was used for rose spraying. Also spraying of willow 2 times at weekly intervals against Melampsora epitea decreased the mean no. of rust pustules per leaf. Spraying of dieffenbachia 24 h before inoculation with M. roridum resulted in significant decrease of necrosis spread on leaves. Five days after inoculation the compds. inhibited the development of leaf spot about 94%. Chitosan applied preventively before inoculation of leaves gave significantly better results in the control of M. roridum than used 24 h after inoculation. Spraying of chrysanthemum, naturally infected with Puccinia horiana decreased the mean no. of pustules per leaf about 95%."

The college is not a secret, its SUNY College of Environmental Science and Forestry in Syracuse, where I am currently finishing my undergrad. I've gotten to know some of the professors working on this project over the years and they have been working very hard on getting this to be a success and are very friendly people. I took a tissue culture course that was taught by some of the people working on the project and got to see firsthand some of the laboratory difficulties and challenges that had to be overcome to make it work. Elm grows like a weed in terms of micropropagation but chestnut is an absolute nightmare! Resin- I beleive the gene used in the chestnut project came from a species of Wheat that just happened to have some sort of natural mechanism for fighting a similar pathogen, and it happened to work. There may in fact be a group of genes being used, I have no formal training in genetics and was never introduced to that aspect of the project. For the elms I beleive this is the case, as there are several different diseases attacking them that needed to be addressed (i.e. elmy yellows, blight). I don't know too much about that project, although as part of the field trials several of the "immune" plants were planted in a group with "baseline" american elms in front of our campus library. they are monitored 24 hours a day for both security and to compare their growth to the normal seedling elms. Only the professors know which is which. Some of the biogengineered elms come from a few plants that were found thriving in the nearby solvay wastebeds in absolutely atrocious, dry, polluted, compacted conditions- the idea being that our trees should be quite urban-tolerant!

Very interesting! I live in Bonney Lake, just one mile away from this area. We had a terrible wind storm a couple months ago, knocking out the power for about 12 hours, and after that, I noticed signs throughout the forest saying, "Danger! Stay out! Wind-damaged area!" I didn't know about the root rot though. It's my understanding from reading local papers, that this area is actually owned by Weyerhauser and that Quadrant, a supposed subsidiary of Weyerhauser, is planning to develop the majority of it, which is very upsetting to us folks here in Bonney Lake. I'm not quite sure how much of it will be maintained as forest, but there are supposed to be residential and commerical developments. Hubby just told me the other day that he heard Costco is going to be part of that.