discussing TC aka tissue culture problems
lindalana 5b Chicago
5 years ago
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Tissue Cultured Plants
Comments (16)As regards to 'mutants', many of the newer hosta varieties are in fact mutants created in TC vats which are multiplied or sale by further TC. This is not to say that there is anything bad about them, as most of the old varieties were mutants created in nature. Mutants occur in TC fairly commonly and are generally rogued out as the plants are raised to production size. A fair number of these are generally kept and evaluated as for improvements over the base stock but most are destroyed in the long run. The reason they occur so commonly is that TC uses such small explants to form each plant that only a very small number of cells must mutate to give a totally mutant plant. If the Big Sky series has a fairly high number of non conforming plants entering the marketplace, then it is a problem of poor control in the growing out step that is at fault, not the TC itself, these should be caught as the plants are being up-sized. George...See MoreWhat species to tissue culture
Comments (15)Hi everyone, Bromadams, you've posted a very interesting topic here and what you are suggesting has a lot of merit, as it could make these beautiful and rare species available to interested growers thereby helping in their preservation. Up until now I personally have looked at tissue cultured plants as just a commercial proposition for the flower trade and a substitute for a bunch of flowers rather than for the keen bromeliad collector. It's true when buying a tissue cultured plant you often finish up with an attractive plant at a reasonable price, but so have the thousands of others growers that bought the same mass produced plant and they don't really cater for the grower who wants something different. At this stage my preference is for seedlings, simply because each one is an individual. True you will get a large percentage of rubbish which needs to be culled, but when you get a good one, it is unique, and you are the sole owner. I don't think anything could give more satisfaction than personally creating a new plant of distinction. Here in Australia we constantly see loads of tissue cultured plants in K-Mart, Bunnings etc. which are usually un-named, or should I say just carrying the name "bromeliad". Surely if a plant's worth tissue culturing it's worthy of the correct name. On the other hand, if the tissue cultured plant was a rare and beautiful species as you're suggesting, it would certainly be worthy of purchase and you would be helping the cause for the preservation of such rare plants. Finally, have you thought about the prospect of tissue culture on a larger scale and exporting them from your country? Looking at your suggesting from an Australian point of view, tissue culture of rare and beautiful species would make them much more accessible in our country as I believe it is much easier to import tissue cultured plants (still in their sterile flasks) than normal live plants due to our strict AQS regulations. If the aim of your suggestion is to really preserve these precious plants, then surely they would have a better chance of survival if they were spread around the world to growers of similar intentions. All the best, Nev. P.S. Ross, What is your society and can anyone join and take part in the seed growing group?...See MoreTissue Culture Kit
Comments (3)It really depends on your definition of cheap and what materials and equipment you already have. The following sites and their links should be of some help as to what is involved. http://www.omnisterra.com/botany/cp/slides/tc/tc.htm http://www.kitchenculturekit.com/index.htm http://www2.carolina.com If you're only propagating a small number of plants the premade media are probably your best bet. I've tried the home made, fertiliser based TC media. They work OK for seed but you really need hormone supplemented media to do mericlone work well. The first webpage recommends Sigma and GIBCO media. Forget sourcing from these guys. Most of the big laboratory suppliers will not supply home users. I'm sure there are others who can supply media but as far as I'm aware you shouldn't have a problem sourcing supplies from Kitchen Culture Kits and or Carolina Biological Supplies. FWIW most cp's do well in 1/2X MS media + whatever hormones and biocides you want to put in(charcoal is generally not needed). Realistically you may be looking at spending a couple of hundred dollars at least in set up and media, especially when you consider the finetuning of your sterile technique. Ongoing costs are of course cheaper. It may end up cheaper to send the plants to a commercial flasking laboratory for propagation (eg an orchid flasking lab). Head over to the GW Orchid Forum and ask if there are any labs they'd recommend. They may seem expensive but they generally work out cheaper than a once off DIY venture....See MoreTissue Cultured Deppea splendens?
Comments (10)Well Ken I am no stranger to horticulture. Been gardening for 24 years now (I'm 29). I wrote on my blog about what you are describing, where 2 becomes 4, becomes 8, etc. I called it the Leap Frog Effect. Indeed yes, I would like to delve into the realm of tissue culture. Many tissue culture companies will also contract to tissue culture plants for you. I can root cuttings and build stock no problem. The fact that the Deppea splendens is extinct in the wild and since I've not seen it offered much was where my two schools of thought came together, I suppose. I also didn't imagine that every plant out there could be tissue cultured. I'm sure there are ways to go about it without spending indecent sums of money. The Deppea is becoming more common now - a year ago they were selling for over $100 a piece on eBay when Annies Annuals was out of stock. Perhaps a year ago shelling out $500 would have meant getting your money back, perhaps not. I suppose the rarity of a plant does not necessarily always mean that it's "difficult" :)...See Morelindalana 5b Chicago
5 years agolindalana 5b Chicago
5 years ago
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