Preprint- resistance to rose rosette and transmission attributes
henry_kuska
6 years ago
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henry_kuska
5 years agoPatty W. zone 5a Illinois
5 years agoRelated Discussions
Rose Rosette Disease (Mary Shelley)
Comments (39)Really....RRV was never used as a control method nor was it introduced from anywhere. It has been around the U.S. since at least the 1940s. Was it likely spread by the use of multiflora as fences and such? Sure. But being mad about that would make as much sense as being mad 20 years from now if we discover that something we can't currently detect that is found on say Knock Outs, causes all other roses to die. Multiflora as a fence was a conservation idea and it works quite well if the person who uses it is willing to maintane it. Have there been things introduced that back fired? Sure. But the list of invasive and devasting ecosystem criters that have come here on their own is a far longer list: Japanese Beetles Fire Ants Raspberry Ants Africanized Bees Cactus Moths Asian Citrus Psylid Mistle toe Water Hyacinth Snake Heads Armored Catfish Formosen Termites etc etc etc. Do Chemicals have a place? Yes. Should they be used exclusively or all the time-probably not but that is your decision. The above linked article was an uninformed paper from 2000 and I haven't been able to find any evidence that people ever used RRV as a control method. He was writing as it showed promise as a possible control method. In case you don't realize how this works currently, they test it extensively in controlled situations prior to using it as a commercial control. They have been testing a parasitic wasp to control fire ants for over a decade and still haven't released them....See MoreResistance to rose rosette virus
Comments (55)I have earlier given a link to another thread where I discuss the results on neighboring roses of applying RoundUp to test roses. Since each thread will scroll off of this forum at a different rate, I feel it is appropriate to put that information into this thread. " To test for the occurrence of root grafting, experiments were conducted using the systemic herbicide Roundup®. Plants that were treated with the herbicide died as expected and approximately 50% of the adjacent, untreated Rosa multiflora ‘Burr’ plants and 10% of the adjacent untreated ‘Dr. Huey’ and ‘Sunflare’ plants showed mild herbicide toxicity symptoms. This could be explained if root grafting had occurred, allowing herbicide to move through the vascular connection between plants. " In the full paper the experimental conditions were given. "The Round-up tests utilized 470 multiflora plants, 148 plants of Dr. Huey, and 98 plants of Sunflare. The multiflora and Dr. Huey were planted approximately 11.8 inches apart (H.Kuska comment: it was not clear to me if they were mixed in the same row or in different rows). The Sunflare plants were planted 3.3 feet apart in rows. Roundup was applied to every other plant. The treated plants all died. About 50% of the multiflora plants showed roundup symptoms. 10 % of the Dr. Huey and 10% of the Sunflare plants showed symptoms." --------------------------------------------------------------------------------------- I would expect that 3.3 feet apart would not be uncommon in many home rose gardens. I cannot post the complete paper because it is copyrighted. However, I can send e-mail copies to a limited number of individual requests under the educational exclusion. My e-mail address is: kuska@neo.rr.com The abstract is at: http://www.actahort.org/members/showpdf?booknrarnr=751_26 -------------------------------------------------------------------------------------- Even the RoundUp label acknowledges that root grafting is a known problem: This is the instruction from the Round-Up label for cut stump applications: "Apply this product using suitable equipment to ensure coverage of the entire cambium. Cut trees or resprouts close to the soil surface. Apply a 50 to 100 percent solution of this product to the freshly cut surface immediately after cutting. Delays in application may result in reduced performance. For best results, applications should be made during periods of active growth and full leaf expansion. PRECAUTIONS, RESTRICTIONS: DO NOT MAKE CUT STUMP APPLICATIONS WHEN THE ROOTS OF ADJACENT DESIRABLE TREES MAY BE GRAFTED TO THE ROOTS OF THE CUT STUMP. INJURY RESULTING FROM ROOT GRAFTING MAY OCCUR IN ADJACENT TREES. Some sprouts, stems, or trees may share the same root system. Adjacent trees having a similar age, height and spacing may signal shared roots. Whether grafted or shared, injury is likely to occur to non-treated stems/trees when one or more trees sharing common roots are treated." http://aggie-horticulture.tamu.edu/syllabi/325/schedule/Pest%20Management/Chemical%20Application%20&%20Safety/Roundup%20Original.pdf --------------------------------------------------------------------------...See Moreevaluation-of-rose-germplasm-for-resistance-to-rose-rosette-disease-a
Comments (6)The link above worked for me. When I find a link that does not work, I often can find the article by putting the title in quotes and putting it into Google. In this case this results in: https://www.google.com/search?num=100&newwindow=1&source=hp&ei=FNPfWq2vI4fusQW-h72QBA&q=%22evaluation-of-rose-germplasm-for-resistance-to-rose-rosette-disease-a%22&oq=%22evaluation-of-rose-germplasm-for-resistance-to-rose-rosette-disease-a%22&gs_l=psy-ab.12...1664.16166.0.18919.4.3.0.0.0.0.127.317.1j2.3.0....0...1.1j2.64.psy-ab..1.1.126.0..35i39k1.0.4DcrZxeltkQ I have a copy of the thesis, but it is copyrighted so I cannot post it. If you have a specific question about it, I can answer the question by putting small sections in quotes. "Table of contents: Chapter 1 INTRODUCTION : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : page 1 - 22 2 SCREENING OF ROSA GERMPLASM FOR RESISTANCE TO ROSE ROSETTE DISEASE UNDER FIELD CONDITIONS : : : page 23 - 45 3 GRAFT AND MECHANICAL TRANSMISSION STUDIES OF ROSE ROSETTE VIRUS : : : : : : : : : : : : : : : : : : : : : : : : : page 46 - 54 4 BIOLOGICAL CONTROL OF PHYLLOCOPTES FRUCTIPHILUS (ACARI: ERIOPHYIDAE) USING PREDATORY MITES FROM THE FAMILY PHYTOSEIIDAE (ACARINA: MESOSTIGMATA) : : : : : : : : : : : : : : : : : : : : pages 55 - 69 Appendix A WEEKS ROSES LETTER TO CUSTOMERS : : : : : : : : : : : : page 70 B ENDPOINT RT-PCR PROTOCOL TO DETECT ROSE ROSETTE EMARAVIRUS : : : : : : : : : : : : : : : : : : : : : : : : page 71 -72 LIST OF TABLES 2.1 Genotypes in this study that have existing resistance data : : : : : page 26 2.2 Results from resistance screening for rose rosette disease, including the number of plants infected and number planted in the field. : : : page 32 -38 2.3 Ordinary run analysis (Madden et al. 1982) : : : : : : : : : : : : : page 41 B.1 Primers used in two-step endpoint RT-PCR protocol : : : : : : : : page 73"...See MorePh.D. thesis - rose rosette virus
Comments (1)Please particularly notice the section about temperature dependence. Pages 131-132. "One trend that was noted throughout all project objectives is the tendency of RRV titer to go below detectable levels in the hot Texas summer months. This change is accompanied by little or no symptom expression and improvements in plant health. Upon further research, it is proposed that a “heat masking” phenomenon (Szittya, et al., 2003) is occurring in summer months (May-August). There are several instances of this effect occurring throughout the project. In field trials, when roses would be PCR tested in March, many times the plants would yield Ct values in the strong positive range for RRV. However, when tested in August or November, very few of the roses would yield a positive result. In the same set of experiments, light RRD symptoms would occur in cooler months (November-March), but would disappear when the temperature began to rise. In transmission experiments, the same effect was noticed, however it was more extreme due to much higher temperatures in greenhouses. The differences in RRV has also been noted in primer sets. I have observed that the RRV(db) primer set (Di Bello, et al., 2017) tends be more sensitive in samples taken in August-March, whereas the primer set RRV2 (Dobhal, et al., 2016) yields more sensitive results on samples in AprilSeptember. While heat masking refers to more external factors, it is also related to changes in RNA silencing (Chung, et al., 2016). In extreme heat, RNA silencing by plants tends to be more effective against viruses, reducing the number of viral particles in plants. RNA silencing is also what provides the heat masking effect as it can allow asymptomatic leaves to emerge at higher temperatures (Chung, et al., 2016). It is proposed that higher temperatures affect a possible suppressor of silencing that is produced by RRV, allowing 132 the host plant’s RNA silencing machinery to be more effective in reducing viral loads in plants. Heat related RNA silencing is also proposed to be part of why known susceptible cultivars of roses remain infected with RRV, yet asymptomatic, for more than 18 months."...See MoreValRose PNW Wa 8a
5 years agolast modified: 5 years agoPatty W. zone 5a Illinois
5 years agoPatty W. zone 5a Illinois
5 years agoValRose PNW Wa 8a
5 years agoValRose PNW Wa 8a
5 years agohenry_kuska
5 years agolast modified: 5 years agohenry_kuska
5 years agojadae15
5 years agoValRose PNW Wa 8a
5 years agohenry_kuska
5 years agoPatty W. zone 5a Illinois
5 years agolast modified: 5 years agohenry_kuska
5 years agohenry_kuska
5 years agoValRose PNW Wa 8a
5 years agojadae15
5 years agohenry_kuska
5 years ago
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